High Throughput Assays for Drug Targets and Enzymes

ProFoldin offers high throughput assays for validated drug targets. The assays are used for tests of inhibition or IC50 measurement of compounds against the specific drug target proteins and enzymes. The assays are in 96-well or 384-well plate format and suitable for high throughput screening (HTS). The validated drug targets include human proteins for anti-cancer and proteins from bacterial, viral and bacterial phage for anti-infection drug discovery.  ProFoldin also provides enzymes, DNA, enzyme substrates and detection dyes separately for drug target protein characterization, assay optimization and HTS needs.

Assays for Anticancer Drug Targets
Human DNA topoisomerase I                                      
Human DNA topoisomerase II                                      
Human PARP-1                                      
Human DNA polymerase alpha                                      
Human DNA polymerase beta                                      
Human DNA polymerase gamma                                      
Human mitochondrial RNA polymerase                                      
Human kinases                                      
Human mitochondrial ClpP protease                                      
Assays for Antibacterial Drug Targets  
Bacterial DNA topoisomerase I  (E. coli Topoisomerase I)                    

Bacterial DNA gyrases 

Quinolone-resistant  mutant E. coli gyrases

(E. coli, S. aureus, P. aeruginosa gyrases)          
Bacterial DNA topoisomerase IV  (E. coli, S. aureus, S. pneumoniae, P. aeruginosa topo IV)
Bacterial DNA polymerases  (E. coli, H influenzae, S. pneumoniae DNA Polymerase III)
Bacterial RNA polymerases  (E. coli, S. aureus RNA polymerases, PNPase)              
Bacterial DNA helicases  (E. coli, H. influenzae, S. pneumoniae, P. aeruginosa DnaB)
Bacterial DNA primases  (E. coli, H. influenzae, S. pneumoniae, S. aureus DnaG)
Bacterial DNA ligases  (E. coli ligase)                            
Bacterial kinases  (NAD Kinase, TMK, UMP Kinase, Guanylate kinase)
Bacterial beta lactamase (Bacterial beta lactamases)                                    
Bacterial cell wall synthesis (MurA, MurC, MurD, MurE, MurF enzymes)          
Bacterial Ddl (E. coli D-alanine—D-alanine ligase)              
Bacterial GlmU  (E. coli UDP-N-acetylglucosamine pyrophosphorylase)  
Bacterial IspD (E. coli, P. aeruginosa methylerythritol phosphate cytidyltransferases)                    
Bacterial PncB (S. aureus, S. pneumoniae nicotinate phosphoribosyltransferases)
Bacterial biotin protein ligase  (E. coli BirA)                            
Proteases  (Trypsin, chymotrypsin, proteinase K)            
(M. Tuberculosis ClpP1P2)                                    
Assays for Antiviral and Bacteriophage Drug Targets
Bacteriophage T7 RNA polymerase