Human Mitochondrial RNA Polymerase Assay

The human mitochondrial RNA polymerase (h-mtRNAP or POLRMT) functions as the transcriptase for expression and the primase for replication of mitochondrial DNA. It’s malfunction is related to various diseases and aging. Some antiviral nucleoside analogs were reported to cause chronic toxicity and related to inhibition of mitochondrial RNA polymerase activity. The Human Mitochondrial RNA Polymerase Assay is based on measurement of the RNA molecules synthesized by the RNA polymerase using a DNA template and DNTPs.  It is fluorescence assay in a 384-well or 96-well plate format. The assay can be used for measurement activities of human mitochondrial RNA polymerase and drug screens against this enzyme.

The Human Mitochondrial RNA Polymerase Assay Kit (Catalog No. MRPA100K) includes all the assay kit components except the enzyme for 100 assays in a 384-well plate assay format: 400 µl of 10 x Buffer, 33 µl of 100 x DNA template, 33 µl of 100 x NTP mix, 330 µl of 10 x fluorescence dye.

The Human Mitochondrial RNA Polymerase Assay Kit Plus (Catalog No. MRPA100KE) includes all the assay kit components for 100 assays in 384-well plate assay format: 400 µl of 10 x Buffer, 33 µl of 100 x DNA template, 33 µl of 100 x Human Mitochondrial RNA Polymerase, 33 µl of 100 x NTP mix, 330 µl of 10 x fluorescence dye.