DNA ligase assay kits

NAD⁺-dependent DNA ligases are present in bacteria, some entomopox viruses and mimi virus. Since NAD⁺-dependent DNA ligases are essential for bacterial growth, they are valuable targets for identifying novel antibacterial agents. The NAD⁺-dependent DNA Ligase Assay Kit is to measure the DNA ligase product in which the diphosphodiester bond is formed at the single stand break of a duplex DNA substrate. The ligase reaction is monitored by the fluorescence intensity at 535 nm. The assay is in 96-well plate format and can be used for screening inhibitors of DNA bacterial ligases.

NAD⁺-dependent DNA Ligase Assay Kit (enzyme not included) (Catalog number NLA100K)

Each kit includes the assay buffer, DNA substrate, Reagent T and the fluorescence dye for 100 assays. Reagent T is used to stop the reaction and denature the DNA at the end of the ligase reaction. Ligase is not included in the kit.

E. coli NAD⁺-dependent DNA Ligase Assay Kit Plus (enzyme included) (Catalog number NLA100KE)

Each kit includes the assay buffer, DNA substrate, Reagent T, E. coli NAD⁺-dependent DNA ligase and the fluorescence dye for 100 assays. Reagent T is used to stop the reaction and denature the DNA at the end of the ligase reaction.

References:

Chiriac AI et al, Mode of action of closthioamide: the first member of the polythioamide class of bacterial DNA gyrase inhibitors. J. Antimicrob. Chemother. 70 (9): 2576-2588 (2015).