Human DNA polymerase theta (Pol Q) encoded by the POLQ gene plays a key role in the DNA repair process. Pol qis overexpressed in various cancers and is a validated anti-cancer drug target.  It is a multiple-functional protein including an N-terminal ATPase-helicase domain and a C-terminal DNA polymerase domain.  The human DNA polymerase theta ATPase assay is based on the DNA-dependent ATPase activity of the N-terminal ATPase-helicase domain. It is a colorimetric assay by detection of phosphate at the wavelength at 650 nm. The assay is performed in a 384-well or 96-well plate format. The assay can be used for detection of the DNA-dependent ATPase activity and inhibitors of human DNA polymerase theta.

The helicase activity assay of human DNA polymerase theta is also available. Please click here.

The Human DNA Polymerase Theta ATPase Assay Kit (Catalog No.  HDPQ100K) includes all the assay kit components except the enzyme for 100 assays in a 384-well plate assay format: 400 µl of 10 x Buffer, 33 µl of 100 x DNA (20 µM), 33 µl of 100 x ATP (25 mM) and 5 ml of MPA3000 dye. The kit does not include the enzyme.

The Human DNA Polymerase Theta ATPase Assay Kit Plus (Catalog No.  HDPQ100KE) includes all the assay kit components for 100 assays in a 384-well plate assay format: 400 µl of 10 x Buffer, 33 µl of 100 x DNA (20 µM), 33 µl of 100 x ATP (25 mM), 33 µl of 100 x human DNA polymerase theta N-terminal domain and 5 ml of MPA3000 dye.