Human DNA polymerase beta assay

 

DNA polymerase beta play key roles in the base excision repair (BER) process.  It fills the small gaps (1 – 6 bases) of one DNA stand using the complementary strand as a template.  Human DNA polymerase beta is an anti-cancer target.  The human DNA polymerase beta assay is based on the principle that the repaired DNA forms more stable DNA duplex than the un-repaired one.  The Assay Kit is to measure the formation of the repaired DNA by its fluorescence signal in the presence of a fluorescence dye.

Each Human DNA Polymerase Beta Assay Kit (Catalog No: DPB100K)  includes 800 µl of 10 x Buffer BP, 55 µl of 100x DNA template, 55 µl of 100 x dNTP mix, 22 ml of Reagent U and 420 µl of 10 x fluorescence dye for 100 assays of human DNA polymerase reactions in a 96-well plate format. The kit does not include human DNA polymerase beta.

Each Human DNA Polymerase Beta assay Kit Plus (Catalog No: DPB100KE) includes 800 µl of 10 x Buffer BP, 55 µl of 100x DNA template, 55 µl of 100 x dNTP mix, 22 ml of Reagent U, 52 µl of 100 x human DNA polymerase beta and 420 µl of 10 x fluorescence dye for 100 assays of human DNA polymerase reactions in a 96-well plate format

 

References:

  1. Merlo S et al, Identification of 5‑Methoxyflavone as a Novel DNA Polymerase-Beta Inhibitor and Neuroprotective Agent against Beta-Amyloid Toxicity. J. Nat. Prod. 78, 2704−2711 (2015).
  2. Maldonado E et al, Expression, purification, and biochemical characterization of recombinant DNA polymerase beta of the Trypanosoma cruzi TcI lineage: requirement of additional factors and detection of phosphorylation of the native form, Parasitol Res (2015) 114:1313–1326.