Topoisomerase assay kit
Human topoisomerase II – DNA decatenation assay

Human DNA topoisomerase II alpha converts the concatenated DNA into decatenated DNA. ProFoldin offers the Human DNA topoisomerase II assays in two assay formats: Spin-column-based DNA decatenation assay and 96-well filter plate–based DNA decatenation assay. In the spin-column-based DNA decatenation assay, the decatenated DNA is separated from the concatenated DNA by a quick and easy spin-column process. The concatenated DNA stays on the column, while the decatenated DNA is eluted. The eluted DNA is quantified by fluorescence.  In the 96-well filter plate–based DNA decatenation assay, the decatenated DNA is separated from the concatenated DNA by a filtration process.  The decatenated DNA passed through the filter (TDD filter plate), received in a 96 well plate and quantified by fluorescence at 535 nm (excitation at 485 nm).

Spin-column-based human topoisomerase II DNA decatenation assay kits Catalog No.
HDC020KE
HDC100KE
HDC020K
HDC100
   
96-well filter plate – based or gel-based human topoisomerase II DNA decatenation assay kits Catalog No.

96-Well Human Topo II DNA Decatenation Assay Kit Plus

Gel-based Human Topoisomerase II DNA Decatenation Assay Kit Plus -50 

HDD96KE

HDD50KEG

 

References:

  1. Merzouki A et al, Adva-27a, a Novel Podophyllotoxin Derivative Found to Be Effective against Multidrug Resistant Human Cancer Cells, Anticancer Research 32: 4423-4432 (2012).
  2. Frank NE et al, Comparative effects of doxorubicin and a doxorubicin analog, 13-deoxy, 5-iminodoxorubicin (GPX-150), on human topoisomerase IIβ activity and cardiac function in a chronic rabbit model. Invest New Drugs.34:693 (2016).