Human topoisomerase II – DNA decatenation assay

Human DNA topoisomerase II alpha converts the concatenated DNA into decatenated DNA. The assay Kit is based the principle that the decatenated DNA is separated from the concatenated DNA by a quick and easy spin-column process. The concatenated DNA stays on the column, while the decatenated DNA is eluted. The eluted DNA is quantified by fluorescence.

 
For 20 assays, enzyme is included.
 
For 20 assays, enzyme is not included.
 
For 100 assays, enzyme is included.
 
For 100 assays, enzyme and kDNA are not included.