The primary biological function of bacterial DNA topoisomerase IV (the ParC-parE complex) enzymes is DNA decatenation. Bacterial Topo IV enzymes also have DNA relaxation and ATPase activities. Some inhibitors such as ciprofloxacin cause DNA damage by DNA cleavage (topoisomerase poisons). ProFoldin offers all the assays for various biological functions of DNA topoisomerase IV enzymes and the DNA cleavage assay of topoisomerase poison inhibitors. The DNA decatenation assays are carried out in a simple spin-column format or a high throughput 96-well plate format. For gel-based assay, please click here.

Bacterial topo IV DNA decatenation assays in spin-column format

DNA Topoisomerase IV Assay Kit (Cat. No: DDC020K):  20 DNA decatenation assays, enzyme is not included.

DNA Topoisomerase IV Assay Kit Plus   (Cat. No: DDC020KE): 20 E. coli Topo IV DNA decatenation assays.

DNA Topoisomerase IV Assay Reagent Set (Cat. No: DDC100): 100 DNA decatenation assays; enzyme or DNA is not included.

DNA Topoisomerase IV Assay Reagent Set Plus (Cat. No: DDC100KE): 100 E. coli Topo IVDNA decatenation assays.

Bacterial topo IV DNA decatenation assays in 96-well plate filter format

96-well Topoisomerase DNA Decatenation Assay Kit (Cat. No: TDD96K): 96 DNA decatenation assays; enzyme is not included.

96-well E. coli Topo IV DNA Decatenation Assay Kit Plus (Cat. No: EDD96KE):     96 E. coli topo IV DNA decatenation assays.  

96-well S. aureus Topo IV DNA Decatenation Assay Kit Plus (Cat. No: SDD96KE): 96 S. aureus topo IV  DNA decatenation assays.

96-well S. pneumoniae Topo IV DNA Decatenation Assay Kit Plus (Cat. No: NDD96KE): 96 S. pneumoniae topo IV DNA decatenation assays.

96-well P. aeruginosa Topo IV DNA Decatenation Assay Kit Plus (Cat. No: PDD96KE):   96 P. aeruginosa topo IV DNA decatenation  assays  .

Bacterial topo IV ATPase assays

E. coli Topo IV ATPase Assay Kit Plus (Cat. No: T4A-100KE):  100 E. coli topo IV ATPase assays.

S. pneumoniae Topo IV ATPase Assay Kit Plus (Cat. No: T4A-100KN):  100 S. pneumonia topo IV ATPase assays.

P. aeruginosa Topoisomerase IV ATPase Assay Kit Plus (Cat. No: T4A-100KP):  100 P. aeruginosa  topo IV ATPase assays.

S. aureus Topoisomerase IV ATPase Assay Kit Plus   (Cat. No: T4A-100KS): 100 S. aureus topo IV ATPase assays.

Bacterial topo IV DNA cleavage assays

96-well Topoisomerase DNA Cleavage Assay Kit (Cat. No: TDC96K): 96 DNA cleavage assays; topoisomerase is not included.

96-well E. coli Topo IV DNA Cleavage Assay Kit Plus (Cat. No: T4C96KE): 96 E. coli topo IV DNA cleavage assays.

96-well S. aureus Topo IV DNA Cleavage Assay Kit Plus (Cat. No:: T4C96KS):  96 S. aureus topo IV DNA cleavage assays.

High throughput topo IV DNA relaxation assays

E. coli Topo IV DNA Relaxation Assay Kit Plus (Cat. No:T4RA-100KE): 100  E. coli topo IV DNA relaxation assays.

S. pneumoniae Topo IV DNA Relaxation Assay Kit Plus (Cat. No:T4RA-100KN): 100 S. pneumonia topo IV DNA relaxation assays.

P. aeruginosa Topo IV DNA Relaxation Assay Kit Plus (Cat. No: T4RA-100KP): 100 P. aeruginosa topo IV DNA relaxation assays.

S. aureus Topo IV DNA Relaxation Assay Kit Plus (Cat. No:T4RA-100KS): 100 S. aureus topo IV DNA relaxation assays.

Reference:                               

1. Narayanan S et al, A cell cycle-controlled redox switch regulates the topoisomerase IV activity. Genes Dev. 29(11):1175-87 (2015).

2. Ushiyama F. ert al, Lead Identification of 8‑(Methylamino)-2-oxo-1,2-dihydroquinoline Derivatives as DNA Gyrase Inhibitors: Hit-to-Lead Generation Involving Thermodynamic Evaluation. ACS Omega 5, 10145−10159 (2020).