Topoisomerase assay kit
DNA gyrase– DNA supercoiling assay

 

DNA topoisomerases such as bacterial topoisomerase II (gyrase) convert relaxed circular DNA into supercoiled DNA.  The DNA Topoisomerase II (Gyrase) Assay Kit is based on the principle that the supercoiled DNA and relaxed DNA yield different fluorescent intensity when interact with fluorescence dye H19.  The relaxed DNA suppresses the fluorescent intensity much more than the supercoiled DNA.  Therefore, when the relaxed DNA is converted into its supercoiled form, the fluorescent signal increases.  The change of fluorescence intensity is used to measure the supercoiling reaction.

Bacterial gyrase DNA supercoiling assays

H19 Dye for DNA Relaxation and Supercoiling Assays (Catalog number: DSA1000D)

DNA Gyrase Assay Kit (Catalog No. DSA020K)

E. coli DNA Gyrase Assay Kit Plus (Catalog No. DSA020KE)

E. coli DNA Gyrase Assay Kit Plus-100 (Catalog No. DSA100KE)

S. aureus Gyrase DNA Supercoiling Assay Kit Plus (Catalog No. DSA020KSE)

S. aureus Gyrase DNA Supercoiling Assay Kit Plus-100 (Catalog No. DSA100KSE)

P. aeruginosa Gyrase DNA Supercoiling Assay Kit Plus (Catalog No. DSA100KP)

 

Bacterial gyrase ATPase assays

E. coli gyrase ATPase assay Kit Plus (Catalog No. T2A-100KE)

S. aureus gyrase ATPase assay Kit Plus(Catalog No. T2A-100KS)

P. aeruginosa Gyrase ATPase assay Kit Plus (Catalog No. T2A-100KP)

 

Bacterial gyrase DNA cleavage assays

96-well E. coli Gyrase DNA Cleavage Assay Kit Plus (Catalog No. T2C96KE)

96-well Topoisomerase DNA Cleavage Assay Kit (Catalog No. TDC96K)

 
 
Relaxed DNA substrate and gyrases

References:

  1. Asha MK et al, In vitro anti-Helicobacter pylori activity of a flavonoid rich extract of Glycyrrhiza glabra and its probable mechanisms of action, Journal of Ethnopharmacology, Vol.145(2), pp.581-586 (2013).
  2. Mora-Pale M. et al, Antimicrobial mechanism of resveratrol-trans-dihydrodimer produced from peroxidase-catalyzed oxidation of resveratrol. Biotechnol Bioeng.Vol 112, pp2417–2428 (2015).
  3. "Tabrizi MA et al,Synthesis and Biological Evaluation of Pyrazolo[3,4-b]pyridin-4-ones as a New Class of Topoisomerase II Inhibitors. Medicinal Chemistry, 11, 342-353 (2015).
  4. Khan UG, Developing a Lead Compound for the Inhibition of Bacterial DNA Gyrase. University of Toronto, Dissertations Publishing, (2016). 10192322.