Human topoisomerase I – DNA relaxation assay

Home

Protein preparation

Bioanalysis

Enzyme assays

Binding assays

How to order

Literature

Human topoisomerase I – DNA relaxation assay

Human topoisomerase I DNA relaxation assay kit (Catalog No. HRA020K)

Human topoisomerase I DNA relaxation assay HTS kit (Catalog No. HRA1000)

Human topoisomerase I relaxes supercoiled plasmid DNA to form relaxed DNA. The Human DNA topoisomerase I assay is based on the principle that the supercoiled DNA and relaxed DNA yield different fluorescent intensity when interact with fluorescence dye H19. The relaxed DNA suppresses the fluorescent intensity much more than the supercoiled DNA. Therefore, when the supercoiled DNA is converted into its relaxed form, the fluorescent signal decreases. The change of fluorescence intensity is used to measure the relaxation reaction.

Each Human topoisomerase I DNA relaxation assay kit (Catalog No. HRA020K) includes the reaction buffer, supercoiled plasmid DNA and fluorescence dye H19 for 20 DNA relaxation reaction assays in a 96-well plate or 40 assays in a 384-well plate. The reaction buffer is optimized for huamn topoisomerase I.

The Human topoisomerase I DNA relaxation assay HTS kit (Catalog No. HRA1000)

is designed for drug discovery HTS uses. Each reagent set includes the reaction buffer and fluorescence dye H19 for 1000 assays of DNA relaxation reactions in a 96-well plate assay format or 2000 assays in a 384-well assay format. Supercoiled DNA is not included in the set. Supercoiled plasmid pBR322 from any commercial source can be used.