Located in Massachusetts, USA and established in 2005, ProFoldin has been providing innovative and unique research products and services focusing on the following areas:

 

ProFoldin’s products and services are broadly employed by universities, research institutes, biotech companies, pharmaceutical R&D and research hospitals worldwide in the laboratories of life sciences and drug discovery. 

Publications:

  1. Kuznetsova V. E. et al. Genome-wide Analysis of Substrate Specificities of the Escherichia coli Haloacid Dehalogenase-like Phosphatase Family,  J. Biol. Chem. VOL.  281, NO. 47, pp. 36149–36161 (2006).  [Protein folding columns, SFC01-10 and PFC].

  2. Cha J. et al. Characterization of the β-Lactam Antibiotic Sensor Domain of the MecR1 Signal Sensor/Transducer Protein from Methicillin-Resistant Staphylococcus aureus,  Biochemistry, 46(26), pp 7822–7831 (2007). [Protein folding columns, SFC01-10 and PFC].

  3. Hao X. et al. Overexpression and Purif ication of Membrane Transport Protein with Native Functions,  Chinese J. Bioch. Mol. Biol. 23:12, 1051-1058 (2007). [Protein folding columns, SFC01-10 and PFC].

  4. Thomas J. et al. Acyl Carrier Protein Phosphodiesterase (AcpH) of Escherichia coli Is a Non-Canonical Member of the HD Phosphatase/Phosphodiesterase Family  Biochemistry, 46, 129-136 (2007). [Protein folding columns, SFC01-10 and PFC].

  5. Cha J. et al. Lysine N-Decarboxylation in the BlaR1 Proteinfrom Staphylococcus Aureus at the Root of Its Function As an Antibiotic SensorJ. Am. Chem. Soc., 129 (13), pp 3834–3835 (2007). [Protein folding columns, SFC01-10 and PFC].

  6. Pelletier D.M, Hemoglobin binding protein from Actinobacillus pleuropneumoniae: a novel method for extraction and isolation. McGill University, Montreal Q.C. Graduate thesis, (2007). [Membrane protein folding columns, MFC01-20].

  7. 谢浩,等,膜转运蛋白的功能性超表达和纯化(Overexpression and Purification of Membrane Transport Protein with Native Functions) , 中国生物化学与分子生物学报(Chinese Journal of Biochemistry and Molecular Biology, 23 (12):1051 (2007)。[Membrane protein folding columns, MFC01-20].

  8. Lee S. et al, Cloning, Solubilization, and Characterization of Squalene Synthase from Thermosynechococcus elongatus BP-1, J. Bacteriol., 190: 3808-3816 (2008). [Membrane protein folding columns, MFC01-20].

  9. Kuznetsova E. , Activity-based functional annotation of unknown proteins: HAD-like hydrolases, University of Toronto, Graduate thesis, (2009). [Protein folding columns, SFC01-10 and PFC].

  10. Miller P. S., Kinetic and Structural Studies on the First Three Enzymes in Bacillus Anthracis Isoprenoid Biosynthesis, ProQuest LLC, Ann Arber, P133 (2009). [Protein folding columns, SFC01-10 and PFC].

  11. Arora S. et al, Downregulation of XPF–ERCC1 enhances cisplatin efficacy in cancer cells, DNA Repair, Volume 9, Issue 7, Pages 745-753 (2010). [DNA binding plates, NBP96B].

  12. Huntley J., Considerations for Recombinant Protein Expression and Purification, Southwestern Medical Center, seminar, http://www.utmb.edu/wrce/Huntley%20slides.pdf. [Membrane protein folding columns, MFC01-20].

  13. 谢浩,等, 去垢剂在膜蛋白研究中的应用(Applications of Detergents in Membrane Proteins Research). 生物技术通报(BIOTECHNOLOGY BULLETIN) 2: (2010). [Membrane protein folding columns, MFC01-20].

  14. Siegmund V. et al, Screening mutant libraries of T7 RNA polymerase for candidates with increased acceptance of 2'-modified nucleotides, Chem. Commun., 48, 9870-9872 (2012). [T7 RNA polymerase assay kit, T7RPA100K].

  15. Duk-Hee  K.et al, Novel dual-binding function of a poly (C)-binding protein 3, transcriptional factor which binds the double-strand and single-stranded DNA sequence, Gene, 501 (1), p.33-38, (2012).[Protein folding columns,SFC01-10 and PFC].

  16. Copeland D.L., Production of Recombinant Carp Leptin And its Effects on Lipid Metabolism in the Common Carp (Cyprinus Carpio), Thesis, The University of Akron (2012). [Protein folding columns, SFC01-10].

  17. Morrow K.J. The New Generation of Antibody Therapeutics:Current Status and Future Prospects,Insight Reports, Cambridge Healthtech Institute, Page 84, (2012) [Protein folding columns, SFC01-10].

  18. Merzouki A. et al, Adva-27a, a Novel Podophyllotoxin Derivative Found to Be Effective against Multidrug Resistant Human Cancer Cells, Anticancer Research 32: 4423-4432 (2012). [96-Well Human Topo II DNA Decatenation Assay Kit, HDD96KE].

  19. Choi J.Y. et al, Structure Guided Development of Novel Thymidine Mimetics targeting Pseudomonas aeruginosa Thymidylate Kinase: from Hit to Lead Generation,  J Med Chem.  January 26; 55(2): 852–870 (2012). [Human Thymidylate Kinase Assay Kit, HTMK500KE].

  20.  Arora S., Identifying and Targeting Cellular Mechanisms to Enhance Cisplatin Chemotherapeutic Response in Cancer.  PhD dissertation, The University of Toledo (2012). [DNA binding plates, NBP96B].

  21. Nakano T., et al, Translocation and Stability of Replicative DNA Helicases upon Encountering DNA-Protein Cross-links, J. Biol. Chem. 288: 4649-4658 (2013). [DnaB helicase, DNAB-100EC].

  22. Bonander N. et al, Production, purification and characterization of recombinant, full-length human claudin-1, PLoS One. 8(5):e64517 (2013). [Membrane protein extraction kit, MPE08].

  23. Kang D.H., Novel function of the poly(c)-binding protein α-CP2 as a transcriptional activator that binds to single-stranded DNA sequences, International Journal of Molecular Medicine, Vol 32 (5): 1187-1194 (2013). [Protein folding columns, SFC01-10].

  24. Asha M.K. et al, In vitro anti-Helicobacter pylori activity of a flavonoid rich extract of Glycyrrhiza glabra and its probable mechanisms of action,Journal of Ethnopharmacology, Vol.145(2), pp.581-586 (2013). [Gyrase DNA Supercoiling Assay Kit, DSA020KE].

  25. Takahashi N. et al, TRPV4 channel activity is modulated by direct interaction of the ankyrin domain to PI(4,5)P₂. Nat Commun. 26;5:4994 (2014). [Protein stability and aggregation assay kit, PSA200K].

  26. Mano J. et al, Development of a Reference Material of a Single DNA Molecule for the Quality Control of PCR Testing. Anal. Chem., 86 (17), pp 8621–8627 (2014). [Phosphate assay kit, MPA3000].

  27. Achbergerová.L et al, Degradation of polyphosphates by polyphosphate kinases from Ruegeria pomeroyi. Biotechnology Letters. Volume 36, Issue 10, pp 2029-2035 (2014). [Polyphosphate assay kit, PPD1000].

  28. Welsh MJ et al,  Transgenic pig models of cystic fibrosis, US patent 20140255975A1, Sep 11, 2014. [Membrane protein extraction kit, MPE02].

  29. Zhang J. et al, A Fast Sensor For in Vivo Quantification of Cytosolic Phosphate in Saccharomyces Cerevisiae, Biotechnol Bioeng.Vol 112, pp 1033-1046 (2015). [Polyphosphate assay kit, PPD1000].

  30. Narayanan S. et al,A cell cycle-controlled redox switch regulates the topoisomerase IV activity. Genes Dev. 29(11):1175-87 (2015). [DNA decatenation assay kit, DDC100KE].

  31. Siricilla S. et al, Discovery of a capuramycin analog that kills nonreplicating Mycobacterium tuberculosis and its synergistic effects with translocase I inhibitors. The Journal of Antibiotics, 68, 271–278 (2015). [RNA polymerase assay kits, RPA100KE and RPA100KSE].

  32. Sobhanifar S. et al, Structure and mechanism of Staphylococcus aureus TarM, the wall teichoic acid α-glycosyltransferase, PNAS, 112 (6) E576-E585 (2015). [UDP assay kit, MUD100K].

  33. Ohnishi T et al, Na, K-ATPase α3 is a death target of Alzheimer patient amyloid-β assembly. Proc Natl Acad Sci U S A. 2015 Aug 11;112(32):E4465-74. [Phosphate assay kit, MPA3000].

  34. Mora-Pale M. et al, Antimicrobial mechanism of resveratrol-trans-dihydrodimer produced from peroxidase-catalyzed oxidation of resveratrol. Biotechnol Bioeng.Vol 112, pp2417–2428 (2015). [Gyrase DNA Supercoiling Assay Kit, DSA100KE].

  35. Tabrizi MA et al,Synthesis and Biological Evaluation of Pyrazolo[3,4-b]pyridin-4-ones as a New Class of Topoisomerase II Inhibitors. Medicinal Chemistry, 11, 342-353 (2015). [Gyrase DNA Supercoiling Assay Kit, DSA100KE].

  36. Merlo S., et al, Identification of 5‑Methoxyflavone as a Novel DNA Polymerase-Beta Inhibitor and Neuroprotective Agent against Beta-Amyloid Toxicity. J. Nat. Prod. 78, 2704−2711 (2015). [Human DNA Polymerase Beta Assay Kit, DPB100K].

  37. Maldonado E. et al, Expression, purification, and biochemical characterization of recombinant DNA polymerase beta of the Trypanosoma cruzi TcI lineage: requirement of additional factors and detection of phosphorylation of the native form, Parasitol Res (2015) 114:1313–1326. [Human DNA Polymerase Beta Assay Kit, DPB100K].

  38. Hou Z. et al, Selective in vivo and in vitro activities of 3,3'-4-nitrobenzylidene-bis-4-hydroxycoumarin against methicillin-resistant Staphylococcus aureus by inhibition of DNA polymerase III. Sci Rep. 2015 Sep 1;5:13637. [Bacterial DNA Polymerase Assay Kit, DPA10KN].

  39. Srinivasan S. et al, Hypoxia-induced expression of phosducin-like 3 regulates expression of VEGFR-2 and promotes angiogenesis. Angiogenesis 18:449–462 (2015).[Protein stability and aggregation assay kit, PSA200K].

  40. Sawanta A. et al, Role of mismatch repair proteins in the processing of cisplatininterstrand cross-links. DNA Repair 35: 126–136 (2015).[DNA binding plates, NBP96B].

  41. Giacani L., et al, Transcription of TP0126, Treponema pallidum Putative OmpW Homolog, Is Regulated by the Length of a Homopolymeric Guanosine Repeat, Infection and Immunity 83: 2275-2289 (2015). [Membrane protein folding columns, MFC].

  42. Chiriac A. I. et al, Mode of action of closthioamide: the first member of the polythioamide class of bacterial DNA gyrase inhibitors. J. Antimicrob. Chemother. 70 (9):  2576-2588 (2015). [Ligase, primase, gyrase assay kits].

  43. Rahimi, N, Therapeutic and research application of pdcl3. WO 2016044219 A1, Sep 15, 2016 [Protein stability and aggregation assay kit, PSA200].

  44. Morrison K. D.et al, Unearthing the Antibacterial Mechanism of Medicinal Clay: A Geochemical Approach to Combating Antibiotic Resistance. Scientific reports, 6: 19043 (2016). [Nucleic Acid Removal Kit, NAR911].

  45. Lee J. A. et al, Patient derived mutation W257G of PPP2R1A enhances cancer cell migration through SRC-JNK-c-Jun pathway. Scientific Reports, Vol.6, Article number: 27391 (2016). [Phosphate Removal Columns, MPR020].

  46. Dandley E. C. et al, Atomic layer deposition coating of carbon nanotubes with zinc oxide causes acute phase immune responses in human monocytes in vitro and in mice after pulmonary exposure. Particle and Fibre Toxicology, Vol.13: 29 (2016). [NanoMolar Zinc Assay Kit, NZA1000].

  47. Frank N.E. et al. Comparative effects of doxorubicin and a doxorubicin analog, 13-deoxy, 5-iminodoxorubicin (GPX-150), on human topoisomerase IIβ activity and cardiac function in a chronic rabbit model. Invest New Drugs.34:693 (2016). [Human topo II DNA decatenation assay kit, HDC100KE].

  48. Takayama F. et al, Diurnal dynamic behavior of microglia in response to infected bacteria through the UDP-P2Y6 receptor system. Sci Rep. 6: 30006 (2016). [UDP assay kit, MUD100K].

  49. Vega M.C., Advanced Technologies for Protein Complex Production and Characterization. Springer Cham Heidelberg, New York. p148, (2016) [Membrane protein extraction kit, MPE08].

  50. LiS. et al, Acoustofluidic Transfer of Inflammatory Cells from Human Sputum Samples. Anal. Chem., 88 (11), pp 5655–5661 (2016) [DTT assay kit, DTT200].

  51. Bathke1, J. Structural and Functional Characterization of Plasmodium falciparum Nicotinic Acid Mononucleotide Adenylyltransferase. J Mol Biol 428, 4946–4961  (2016) [Phosphate assay kit, MPA3000].

  52. Khan UG, Developing a Lead Compound for the Inhibition of Bacterial DNA Gyrase. University of Toronto, Dissertations Publishing, (2016). 10192322. [Gyrase DNA Supercoiling Assay Kit, DSA100KE].

  53. Igarashi M et al, Kribellosides, novel RNA 5′-triphosphatase inhibitors from the rare actinomycete Kribbella sp. MI481-42F6. The Journal of Antibiotics (Tokyo), (11 January 2017). [Phosphate assay kit, MPA3000].

  54. Lee DS et al, Differential regulation of mouse and human Mu opioid receptor gene depends on the single stranded DNA structure of its promoter and α-complex protein 1. Biomedical Reports (2017) [Protein folding columns, SFC01-12].

  55. Lacriola CJ et al, Inhibition of DNA replication in Staphylococcus aureus by tegaserod. The Journal of Antibiotics. 70, 918–920 (2017). [DnaG assay Kit, AGA100KE].

  56. Carlos Urda et al, Bistratamides M and N, Oxazole-Thiazole Containing Cyclic Hexapeptides Isolated from Lissoclinum bistratum Interaction of Zinc (II) with Bistratamide K. Marine Drugs 2017, 15(7), 209; doi:10.3390/md15070209 . [Human topo I assay Kit, HRA100KE].

  57. Lee D-S et al, Differential regulation of mouse and human Mu opioid receptor gene depends on the single stranded DNA structure of its promoter and α-complex protein 1. Biomedical Reports,Volume 6 Issue 5, pp:532-538 (2017). [Protein folding columns, SFC01-10].    

  58. Ho M.L et al, Silver nanoprism-based paper as ratiometric sensor for extending biothiol detection in serum. New Journal of Chemistry. Oct 30 (2017) [Cysteine assay kit, Cys200].

  59. Kanai M. et al, Benzothiazole compound and medicine containing same. Patent US2017/0197956 A1 Jul 13 (2017). [Protein stability and aggregation assay kit, PSA200].

  60.  Matsuura, S et al; Enzyme Immobilization in Mesoporous Silica for Enhancement of Thermostability. Journal of Nanoscience and Nanotechnology, Volume 18, Number 1, pp. 104-109  (2018) [Protein stability and aggregation assay kit, PSA200].

  61. Wang LJ et al, Increased serum levels of cysteine in patients with schizophrenia: A potential marker of cognitive function preservation. Schizophrenia Research, Volume 192, Pages 391–397 (2018) [Cysteine assay kit, CYS200].

  62. Abdellatif, Khaled R.A. et al, Design, Synthesis and Cytotoxicity Evaluation of New 3, 5-Disubstituted-2-Thioxoimidazolidinones. Anti-Cancer Agents in Medicinal Chemistry, Volume 18, 573 (2018). [Human topo I assay kit, HRA100KE].

  63. Hyoju, Sanjiv, K et al, Oral Polyphosphate Suppresses Bacterial Collagenase Production and Prevents Anastomotic Leak Due to Serratia marcescens and Pseudomonas aeruginosa. Annals of Surgery, Volume 267 - Issue 6 - p 1112 (2018). [Polyphosphate assay kit, PPD1000].

  64. Shu Yang et al, Emetine inhibits Zika and Ebola virus infections through two molecular mechanisms: inhibiting viral replication and decreasing viral entry, Cell Discovery, Volume 4,  Article number: 31 (05 June 2018). [Virus RNA polymerase assay kit, VRT100K].

  65. Loreen R. Stromberg1 et al, Presentation matters: Impact of association of amphiphilic LPS with serum carrier proteins, on innate immune signaling, PLOS One, June 2018. [Detergent CMC assay kit, CMC1000].

  66. Julian Röwe, Inorganic Polyphosphates – Novel Modulators of Immune Response. Dissertation, Johannes Gutenberg University Mainz - Uni Mainz, Germany (2018). [Polyphosphate assay kit, PPD1000].

  67. Kurnia D. et al, Antibacterial Flavonoids against Oral Bacteria of Enterococcus faecalis ATCC 29212 from Sarang Semut (Myrmecodia pendans) and its Inhibitor Activity against Enzyme MurA. Curr Drug Discov Technol. 2018 Aug 27. [MurA assay kit, MURA100KE].

  68. Hyoju, S.K. et al, Oral Polyphosphate Suppresses Bacterial Collagenase Production and Prevents Anastomotic Leak Due to Serratia marcescens and Pseudomonas aeruginosa. Annals of Surgery,   Vol 267, Number 6, Page 1112 (2018). [Polyphosphate assay kit, PPD1000].

  69. Motomura K. et al, Synthetic Phosphorus Metabolic Pathway for Biosafety and Contamination Management of Cyanobacterial Cultivation. ACS Synth. Biol., Vol 7 (9), 2189 (2018). [Phosphate assay kit, MPA3000].

  70. Thalji RK et al,Structure-guided design of antibacterials that allosterically inhibit DNA gyrase Bioorganic & Medicinal Chemistry Letters, March 2019. [Gyrase assay kit, DSA100KE].

  71. Kubicek-Sutherland J.Z. UndersToxins 2019, 11(4), 202tanding the Significance of Biochemistry in the Storage, Handling, Purification, and Sampling of Amphiphilic Mycolactone. Toxins 11(4), 202 (2019). [Detergent CMC assay kit, CMC1000].

  72. Parveen N. et al, Non-pathogenic Borrelia burgdorferi expressing Treponema pallidum TprK and Tp0435 antigens as a novel approach to evaluate syphilis vaccine. Vaccine, Vol 37, (13)1807 (2019). [Membrane protein folding columns, MFC column].

  73. Jouandin P. et al, Lysosomal cystine efflux opposes mTORC1 reactivation through the TCA cycle. BioRxiv April 2019. [Cysteine assay kit, Cys200].

  74. Van Tine BA.  et al, A phase II clinical study of 13-deoxy, 5-iminodoxorubicin (GPX-150) with metastatic and unresectable soft tissue sarcoma. Cancer Med. 8(6):2994-3003 (2019). [Human Topo II assay].

  75. Martinez SR. et al, Identification of the potential biological target of N-benzenesulfonyl-1,2,3,4-tetrahydroquinoline compounds active against gram-positive and gram-negative bacteria. J Biomol Struct Dyn. 23:1-10 (2019). [MurD assay].

  76. Azam M. A., at al, Structure-based virtual screening to identify inhibitors against Staphylococcus aureus MurD enzyme, Structural Chemistry,  https://doi.org/10.1007/s11224-019-01330-z, April (2019).[ MurD assay].