Human DNA Polymerase Gamma Assay

Human DNA polymerase gamma is the only DNA polymerase in human mitochondria and is responsible for the DNA replication, recombination and repairing in human mitochondria. It plays critical roles in mitochondrial diseases and aging. Some antiviral nucleoside analogs were reported to inhibit DNA polymerase gamma after intracellular phosphorylation and cause severe chronic toxicity.  The human DNA polymerase gamma assay is based on measurement of the DNA molecules synthesized by the DNA polymerase.  The assay is performed in a 384-well or 96-well plate format. The assay can be used for detection of DNA polymerase gamma activity and high throughput screen of human DNA polymerase gamma inhibitors.

The Human DNA Polymerase Gamma Assay Kit (Catalog No.  DPG100K) includes all the assay kit components except the enzyme for 100 assays in a 384-well plate assay format: 400 µl of 10 x Buffer, 33 µl of 100 x DNA template, 33 µl of 100 x dNTP  mix, 1550 µl of 2 x Dye, 1550 µl of 50 mM EDTA.

The Human DNA Polymerase Gamma Assay Kit Plus (Catalog No.  DPG100KE) includes all the assay kit components for 100 assays in 384-well plate assay format: 400 µl of 10 x Buffer, 33 µl of 100 x DNA template, 33 µl of 100 x dNTP  mix, 33 µl of 100 x human DNA polymerase gamma,  1550 µl of 2 x Dye, 1550 µl of 50 mM EDTA.