Human poly (ADP-ribose) polymerase-1 (PARP-1)

Poly (ADP-ribose) polymerase-1 (PARP-1) responses to DNA damage and synthesizes poly ADP-ribose (PAR) that is transferred to itself and a variety of acceptor proteins.  Excessive activation of PARP-1 not only depletes NAD+ but also generates toxic PAR that lead to cell death.  PARP-1 is an anti-cancer drug target.  It is also a potential drug target for other diseases such as stroke, ischemia and reperfusion where high-level DNA damage occurs.  The human PARP-1 assay is based on measurement of its product PAR (poly ADP-ribose) that binds to a fluorescent dye and enhances its fluorescent signal. 

The Human Poly (ADP-ribose) Polymerase-1 Assay Kit (catalog number PAR100K) includes 800 µl of 10 x assay buffer, 55 µl of 100x NAD+, 55 µl of 100x DNA and 220 µl of 100 x fluorescence dye for 100 assays of human PARP-1 in a 96-well plate format.  The kit does not include Human PARP-1.

The Human Poly (ADP-ribose) Polymerase-1 Assay Kit Plus (catalog number PAR100KE) includes 800 µl of 10 x assay buffer, 55 µl of 200x NAD+, 55 µl of 100x DNA, 17 µl of 300 x Human PARP-1 and 220 µl of 100x fluorescence dye for 100 assays of human PARP-1 in a 96-well plate format.