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Human poly (ADP-ribose) polymerase-1 (PARP-1)
Poly (ADP-ribose) polymerase-1 (PARP-1) responses to DNA damage and synthesizes poly ADP-ribose (PAR) that is transferred to itself and a variety of acceptor proteins. Excessive activation of PARP-1 not only depletes NAD+ but also generates toxic PAR that lead to cell death. PARP-1 is an anti-cancer drug target. It is also a potential drug target for other diseases such as stroke, ischemia and reperfusion where high-level DNA damage occurs. The human PARP-1 assay is based on measurement of its product PAR that binds to a fluorescent dye and enhances its fluorescent signal. The Assay Kit (catalog number PAR100K) includes the assay buffer, DNA template and fluorescence dye for 100 assays of human PARP-1 in a 96-well plate format.
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