The S. aureus Gyrase DNA Supercoiling Assay Kit -100 (Catalog No. DSA100KS) includes 500 µl of 10 x Buffer T2, 410 µl of 10 x relaxed DNA, 8 µl of 4500 x H19 dye, 450 µl of 10 x ATP, 820 µl of 2 M potassium glutamate and 3000 µl of 10 x H19 dilution buffer. S. aureus gyrase is not included. It is for 100 assays in a 96-well plate format.