The E. coli DNA Topoisomerase II (Gyrase) Assay Kit-100 (Catalog No. DSA100K) includes 600 µl of 10 x Buffer T2, 405 µl of 10 x relaxed DNA, 8 µl of 4500 x H19 dye, 450 µl of 10 x ATP and 3000 µl of 10 x H19 dilution buffer. E. coli gyrase is not included.