The Bacterial DNA Topoisomerase I Assay Kit-100 (Catalog No. DRA100K) includes 480 µl of 10 x Buffer T1, 405 µl of 10 x supercoiled plasmid DNA, 8 µl of 4500 x Dye H19 and 3000 µl of 10 x H19 dilution buffer.  DNA topoisomerase I is not included in the kit.  It is for 100 DNA relaxation assays in a 96-well plate format or 200 assays in a 384-well assay format.