This product includes the 600 ul of assay buffer (Buffer T4), 520 ul of 10 x concatenated DNA, 120 ul of 50 x ATP (10 mM), 550 ul of 0.4 M EDTA, 780 ul of 20 x fluorescence dye, 100 spin columns and 55ul of 100 x E.coli topoisomerase IV (500 nM) for 100 E. coli topoisomerase IV DNA decatenation assays.