Bacterial MurC Assays

MurC or UDP-N-acetylmuramic acid:L-alanine ligase is the first of four paralogous amino acid-adding enzymes in the pathway of peptidoglycan biosynthesis in bacteria. It is an essential enzyme and attractive target for anti-bacterial drug discovery. MurC catalyzes the addition of L-alanine onto the nucleotide precursor UDP-MurNAc generating UDP-MurNAc-L-Ala. The ligation reaction is coupled to the hydrolysis of ATP forming ADP and inorganic phosphate.

The Bacterial MurC Assay is based on measurement of the inorganic phosphate generated from the MurC reaction.  The inorganic phosphate is detected by light absorbance at 650 nm. The assay reactions and detection can be performed by using 384-well or 96-well assay plates. Alternatively, the assay reaction can be carried out in Eppendorf tubes and the signal is measured using a cuvette. The high throughput assay can be used for screening inhibitors of bacterial MurC in drug discovery research.  It may also be used for characterization of bacterial MurC.

E. coli MurC Assay Kit Plus-100               Catalog No. MURC100KE

E. coli MurC Assay Kit Plus-500               Catalog No. MURC500KE

S. pneumoniae MurC Assay Kit Plus-100   Catalog No. MURC100KN

S. pneumoniae MurC Assay Kit Plus-500   Catalog No. MURC500KN