This product includes the reagents for 500 assays in a 384-well plate assay format including 2 ml of 10 x Assay buffer, 0.170 ml of 100 x UDP-MurNAc, 0.170 ml of 100 x L-Ala, 0.170 ml of 100 x ATP , 0.170 ml of 100 x S. pneumoniae MurC (2000 nM) and 25 ml of Dye for phosphate detection.