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Drug Discovery, Drug Delivery, and Biochemical Assays
ProFoldin’s products and services are employed by universities, research institutes, biotech
companies, pharmaceutical R&D and research hospitals worldwide in life sciences and drug discovery.
Viral RNA-dependent RNA Polymerase Assay

The Viral RNA-dependent RNA Polymerase Assay is developed using a RNA polymerase in the Flaviviridae, a family of positive, single-stranded, enveloped RNA viruses. The assay is based on measurement of the RNA molecules synthesized by the RNA polymerase using RNA as a template in the presence of NTPs.  The assay can be performed in a 384-well or 96-well plate format for tests of theenzyme activities of RNA polymerases in the Flaviviridae family and high throughput screening of inhibitors.  

For SARS-CoV-2 RNA polymerase (RdRp) assay, please click here.

The Viral (Flavivirus) RNA-dependent RNA Polymerase Assay Kit – 100 (Catalog No.VRT100K) includes 350 µl of 10 x Buffer, 350 µl of 10 x MnCl2, 33 µl of 100 x Template, 33 µl of 100 x NTPs (50 mM ATP and 50 mM GTP) and 330 µl of 10 x fluorescence dye. It is for 100 assays of virus RNA polymerase in a 384-well plate format. The assay kit includes all reagents except the enzyme.   

 

The Viral ((Flavivirus) RNA-dependent RNA Polymerase Assay Kit – 500 (Catalog No.VRT500K) includes 1550 µl of 10 x Buffer, 1550 µl of 10 x MnCl2, 160 µl of 100 x Template, 160 µl of 100 x NTPs (50 mM ATP and 50 mM GTP) and 1550 µl of 10 x fluorescence dye. It is for 500 assays of virus RNA polymerase in a 384-well plate format. The assay kit includes all reagents except the enzyme.   

 

 

References:                             

1. Shu Yang et al, Emetine inhibits Zika and Ebola virus infections through two molecular mechanisms: inhibiting viral replication and decreasing viral entry, Cell Discovery, Volume 4,  Article number: 31 (05 June 2018).

2. Chen H et al, Antiviral activity of lycorine against Zika virus in vivo and in vitro. Virology, Volume 546, Pages 88-97 (2020).