Vancomycin is a glycopeptide antibiotic that blocks the cell wall synthesis of bacteria. The MicroGram Vancomycin Assay Kit is designed for measurement of low micrograms of vancomycin. The assay is based on increase of fluorescence at 470 nm of the assay reagent in the presence of vancomycin. The assay kit can be used for measurement of vancomycin concentrations in drug discovery, drug development and pharmaceutical samples. The assay is not compatible with biological samples containing amino acids or other molecules or buffers with amines.
The MicroGram Vancomycin Assay Kit (Catalog number VAN100) includes 0.5 ml of 10 x VAN dye, 5.5 ml of Assay buffer, 5 ml of reagent A. It is for measurement of 100 samples using 96-well plates. Cuvettes may also be used for measurements.