UMP Kinase Assays
UMP kinase or uridylate kinase is essential for biosynthesis of nucleic acid. It catalyzes the ATP-dependent phosphorylation of UMP into UDP.
UMP + ATP = UDP + ADP
UMP kinase is an attractive antibacterial target. The UMP kinase assay is based on measurement of ADP generated from the kinase reaction. The assay is fluorescence-based and can be carried out using regular black 96-well or 384-well plates or micro-cuvettes.
E. coli UMP Kinase Assay Kit Plus-100 (Catalog number: UMK100KE)
E. coli UMP Kinase Assay Kit Plus-500 (Catalog number: UMK500KE)