The following RNA polymerase assays in a 384-well plate format are available:

Bacterial RNA polymerase assays

Two types of bacterial RNAP assays are available: one is to measure RNA synthesis, the other is to measure DNA -dependent pyrophosphate formation.

            E. coli RNA polymerase assay: A fluorescence-based assay to measure RNA synthesis by E. coli RNA polymerase using a single strand DNA template.

            E. coli RNA polymerase pyrophosphate assay: A light absorbance-based assay to measure pyrophosphate formation during RNA formation by E. coli RNA polymerase using a double-strand DNA template.

            S. aureus RNA polymerase assay: A fluorescence-based assay to measure RNA synthesis by S. aureus RNA polymerase using a single strand DNA template.

            S. aureus RNA polymerase pyrophosphate assay: A light absorbance-based assay to measure pyrophosphate formation during RNA formation by S. aureus RNA polymerase using a double-strand DNA template.

            E. coli Polynucleotide Phosphorylase (PNPase) Assay: A fluorescence-based assay to measure Poly(A) synthesis by E. coli PNPase using ADP.

References:

1. Siricilla S et al, Discovery of a capuramycin analog that kills nonreplicating Mycobacterium tuberculosis and its synergistic effects with translocase I inhibitors. The Journal of Antibiotics, 68, 271–278 (2015).

2. Meng J et al, Synthesis and biological evaluation of novel N-2,4-dimethoxyphenyl dithiolopyrrolone derivatives as bacterial RNA polymerase inhibitors. Medicinal Chemistry Research volume 29, 1376–1386 (2020).