E. coli Polynucleotide Phosphorylase (PNPase) Assay

Polynucleotide Phosphorylase (PNPase) plays important roles in mRNA processing and degradation in various cells. It has both exonuclease and polymerase activities. The E.coli PNPase assay is based on measurement of the RNA molecules synthesized by E. coli PNPase using an RNA as a template in the presence of NTPs.  The assay can be performed in a 384-well or 96-well plate format for tests of E. coli PNPase activities and high throughput screening of inhibitors.  

The E. coli Polynucleotide Phosphorylase (PNPase) Assay Kit (Catalog No.  PNP100K) includes 400 µl of 10 x Buffer, 33 µl of 100 x Template, 33 µl of 100 x NTPs (50 mM ATP and 50 mM GTP) and 330 µl of 10 x fluorescence dye. It is for 100 assays of E. coli PNPase in a 384-well plate format. The assay kit includes all reagents except the enzyme.   

The E. coli Polynucleotide Phosphorylase (PNPase) Assay Kit Plus (Catalog No.  PNP100KE)  includes all the reagents for 100 assays of E. coli PNPase in a 384-well plate format. The kit includes 400 µl of 10 x Buffer, 33 µl of 100 x RNA Template, 33 µl of 100 x NTPs (50 mM ATP and 50 mM GTP), 330 µl of 10 x fluorescence dye and 33 µl of 100 xE. coli PNPase (25 µM).