Bacterial RNA polymerase – RNA synthesis assay

 

The bacterial RNA polymerase is responsible for biosynthesis of mRNA, tRNA and rRNA in the cells. The RNA Polymerase Assay Kit is based on measurement of the RNA molecules synthesized by the RNA polymerase using a single strand DNA template. The assay can be performed in 96-well plate or 384-well plate format for high throughput screening of RNA polymerase inhibitors.  For bacterial RNAP assays using double strand DNA, please see RNAP pyrophosphate assays.

Each kit includes the assay buffer and DNA template for 100 assays of RNA polymerase reactions in a 384-well assay format. The assay buffer is optimized for E. coli RNA polymerase. Enzyme is not included.
 
The RNA polymerase assay kit including E. coli RNA polymerase.
 
Each kit includes the assay buffer and DNA template for 100 assays of RNA polymerase reactions in a 384-well assay format. The assay buffer is optimized for S. aureus RNA polymerase. Enzyme is not included.
 
The S. aureus RNA polymerase assay kit including S. aureus RNA polymerase.
 

References:

Siricilla S et al, Discovery of a capuramycin analog that kills nonreplicating Mycobacterium tuberculosis and its synergistic effects with translocase I inhibitors. The Journal of Antibiotics, 68, 271–278 (2015).