The assay measures micromolar concentrations of NADPH. NADPH is the reduced form of nicotinamide adenine dinucleotide phosphate (NADP). NADPH is an enzymatic cofactor for many reductases and acts as an electron donor. It is a product of many oxidases using NADP as an electron acceptor. Both NADPH and NADP are involved in many biosynthesis and degradation pathways. The MicroMolar NADPH Assay Kit provides a convenient tool for sensitive detection of NADPH. The assay is based on detection of light absorbance at 590 nm and the assay process is completed within 20 min. It is in a 96-well plate format and can be used for high throughput screening of enzymes involving NADPH. The assay is compatible with most common buffers in biochemistry labs. It is not compatible with NADH or other reducing reagents such DTT.
The MicroMolar NADPH Assay Kit (Catalog No. NADPH100) includes 1 ml of 10x Assay buffer, 0.1 ml of 20 x Reagent A, 0.1 ml of 20 x Reagent B, 0.5 ml of 10x Reagent C and 0.020 ml of 10 mM NADPH. It is for 100 assays in a 96-well plate format.