The assay measures micromolar concentrations of NADH. NADH is the reduced form of nicotinamide adenine dinucleotide (NAD). NADH is an enzymatic cofactor for many reductases and acts as an electron donor. It is a product of many oxidases using NAD as an electron acceptor. Both NADH and NAD are involved in many biosynthesis and degradation pathways. The MicroMolar NADH Assay Kit (NADH100) provides a convenient tool for sensitive detection of NADH. The assay is based on detection of light absorbance at 590 nm and the assay process is completed within 20 min. It is in a 96-well plate format and can be used for high throughput screening of enzymes involving NADH. The assay is compatible with most common buffers in biochemistry labs. It is not compatible with NADPH or other reducing reagents such DTT.
The MicroMolar NADH Assay Kit (Catalog No. NADH100) includes 1 ml of 10x Assay buffer, 0.1 ml of 20 x Reagent A, 0.1 ml of 20 x Reagent B, 0.5 ml of 10x Reagent C and 0.020 ml of 10 mM NADH. It is for 100 assays in a 96-well plate format.