Quinolone-resistant Gyrase Mutant DNA Supercoiling Assays

Quinolone molecules such as ciprofloxacin are commonly used antibiotics that target bacterial DNA topoisomerases.  Mutations S83W and D87G in the gyrA subunit of E.coli gyrase are proven causing quinolone resistance. Solving the drug resistance problem requires discovery of new molecules that inhibit the mutant topoisomerases. TheQuinolone-resistant E. coli Gyrase Mutant DNA Supercoiling Assay Kits are designed to measure the inhibitory activities of molecules against mutant bacterial gyrases. The assays can be used to test new quinolone compounds in drug discovery.

The Quinolone-resistant E. coli Gyrase Mutant S83W Assay Kit Plus-100 (Catalog number S83W100KE) includes all the reagents for 100 assays of E. coli  gyrase mutant S83W DNA supercoiling activity. It includes 600 µl of 10 x Buffer T2, 405 µl of 10 x relaxed DNA, 20 µl of 1500 x H19 dye, 450 µl of 10 x ATP,  3000 µl of 10 x H19 dilution buffer and 50 µl 100 x E.coli  gyrase mutant S83W.

The Quinolone-resistant E. coli Gyrase Mutant D87G Assay Kit Plus-100 (Catalog number D87G100KE) includes all the reagents for 100 assays of E. coli  gyrase mutant D87G DNA supercoiling activity. It includes 600 µl of 10 x Buffer T2, 405 µl of 10 x relaxed DNA, 20 µl of 1500 x H19 dye, 450 µl of 10 x ATP,  3000 µl of 10 x H19 dilution buffer and 50 µl 100 x E.coli  gyrase mutant D87G.