Bacterial MurE Assays
MurE or UDP-MurNAc-tripeptide ligase is the third amino acid-adding enzymes in the pathway of peptidoglycan biosynthesis in bacteria . It is an essential enzyme and attractive target for anti-bacterial drug discovery. MurE catalyses the addition of lysine or meso-diaminopimelic acid (DAP) into the MurD product UDP-MurNAc-dipeptide in bacteria generating the UDP-MurNAc-tripeptide. The ligation reaction is coupled to the hydrolysis of ATP forming ADP and inorganic phosphate.
The Bacterial MurE Assay is based on measurement of the inorganic phosphate generated from the MurE reaction. The inorganic phosphate is detected by light absorbance at 650 nm. The assay reactions and detection can be performed by using 384-well or 96-well assay plates. Alternatively, the assay reaction can be carried out in Eppendorf tubes and the signal is measured using a cuvette. The high throughput assay can be used for screening inhibitors of bacterial MurE in drug discovery research. It may also be used for characterization of bacterial MurE.
E. coliMurE Assay Kit Plus-100 (Catalog No. MURE100KE)
E. coliMurE Assay Kit Plus-500 (Catalog No. MURE500KE)