ProFoldin offers professional high throughput assay development services. The following the milestones and the time needed to complete the service:

Step 1. Feasibility test

Goal:

(1) Design and prove the assay principle

(2) Estimate the assay signal / background ratio

(3) Establish the HTS assay optimization plan

Time needed: One week after materials are available.

Step 2. HTS assay optimization

Goal:

(1) Enzyme concentration optimization

(2) Template optimization for polymerase assays

(3) Substrate concentration optimization

(4) Time dependence

(5) Buffer optimization

(6) Detection dye optimization

(7) Assay window assessment

(8) DMSO effect

(9) Assay linearity

(10) Optimized assay conditions

Time needed: 2 to 3 weeks

Step 3. Assay validation – control inhibitor tests

Goal: IC50 measurement of 1 - 5 control inhibitors 

Time needed: One week.

Where to start?

Please contact ProFoldin at info@profoldin.com for any questions.