Human Cytomegalovirus (HCMV) DNA Polymerase Assay

Human cytomegalovirus (HCMV) or human betaherpesvirus 5 belongs to the viral family of herpesviruses. HCMV encodes for its own DNA polymerase and uses the host RNA polymerase for the transcription of all of its genes.  The HCMV DNA polymerase is a key enzyme in the lytic phase of the infection. The HCMV DNA polymerase assay is based on measurement of the DNA molecules synthesized by the DNA polymerase in the presence of a DNA template and dNTPs.  The assay can be performed in a 384-well or 96-well plate format for tests of theenzyme activities of HCMV DNA polymerases and high throughput screening of inhibitors. 

The Human Cytomegalovirus (HCMV) DNA Polymerase Assay Kit (Catalog No. HCMV100K) includes all the assay kit components except the enzyme. It includes 400 µl of 10 x Buffer, 33 µl of 100 x DNA template, 33 µl of 100 x dNTP, 1550 µl of 2 x Dye and 1550 µl of 50 mM EDTA.

The Human Cytomegalovirus (HCMV) DNA Polymerase Assay Kit Plus -100 (Catalog No. HCMV100KE) includes all the assay kit components.  It includes  400 µl of 10 x Buffer, 33 µl of 100 x DNA template, 33 µl of 100 x dNTP,  33 µl of 100 x HCMV DNA polymerase, 1550 µl of 2 x Dye and 1550 µl of 50 mM EDTA.