The MicroMolar GDP Assay is for measurement of GDP concentrations in a sub-micromolar to low micromolar range. UDP and CDP showed very little background. ADP will also be detected. Nucleoside triphosphates (ATP, UTP, GTP and CTP) and monophosphates (AMP, UMP, GMP and CMP) are not detected.
The GDP assay is based on fluorescence measurement with emission at 535 nm and excitation at 485 nm. It is a high throughput assay using regular black 96-well or 384-well plates. Micro-cuvettes may also be used for detection of the fluorescence signals. The assay can be used to measure contamination of GDP in GTP samples or monitor hydrolysis of GTP. It can also be used for measurement of enzyme activities that generate GDP molecules. The GDP assay is compatible with common reaction buffers with magnesium. It is not compatible with samples containing ADP or high concentrations of DNA or RNA.
MicroMolar GDP assay kit -100 asays (Catalog number: MGD100K): The kit contains 10 x buffer, 100 x MGD reagent 1, 100 x MGD reagent 2 and 10 x fluorescence dye. The assay reagents are sufficient for measurement of 100 samples of GDP using a standard black 384-well plate.
MicroMolar GDP assay kit -500 assays (Catalog number: MGD500K): The kit contains 10 x buffer, 100 x MGD reagent 1, 100 x MGD reagent 2 and 10 x fluorescence dye. The assay reagents are sufficient for measurement of 500 samples of GDP using a standard black 384-well plate.