DOPC Nanodiscs

Nanodiscs of phospholipids may be used for scientific research involving membrane proteins or cell membranes. The DOPC nanodisc are prepared by solubilizing a liposome composed of DOPC : cholesterol : DSPE-PEG2000 (7.5 : 1 : 2.5) using styrene - maleic acid (SMA) copolymers. The SMA to lipid mass ratio is 8:1.  After the liposome solubilization, the SMA 3:1 copolymer yields nanodiscs in an average diameter about 10 nm. The SMA 2:1 copolymer yields nanodiscs in an average diameter about 30 nm.  The total lipid concentration is 5 mg/ml and the SMA copolymer concentration is 40 mg/ml.  The nanodiscs are formed in a buffer composed of 10 mM Tris-HCl, pH 7.6.

10 nm DOPC Nanodisc (Catalog No. ND-OCGS-10) contains 2 ml of 5 mg/ml lipids composed of DOPC : cholesterol : DSPE-PEG2000 (mass ratio: 7.5 : 1 : 2.5)  and 40 mg/ml styrene - maleic acid (SMA) 3:1 copolymer in a buffer composed of  10 mM Tris-HCl, pH 7.6.

30 nm DOPC Nanodisc (Catalog No. ND-OCGS-30) contains 2 ml of 5 mg/ml lipids composed of DOPC : cholesterol : DSPE-PEG2000 (mass ratio: 7.5 : 1 : 2.5)  and 40 mg/ml styrene - maleic acid (SMA) 2:1 copolymer in a buffer composed of  10 mM Tris-HCl, pH 7.6.