The Coenzyme A Assay Kit is for measurement of micromolar concentrations of Coenzyme A (CoA). The assay is based on the principle that the thiol group of CoA interacts with the CAK reagent and enhances the fluorescence intensity at 535 nm (excitation at 485 nm). Acetyl-CoA does not interact with the CAK reagent.
Coenzyme A Assay Kit (Catalog number CAK1000): The assay reagent is sufficient for measurement of 1000 samples using 384-well plates. The assay kit can be used for measurement of other thiols.
The assay is compatible with HEPES buffer, low concentrations of non-ionic detergent (<0.01%), sodium and potassium salt, MgCl2 (< 5 mM), CaCl2 (<5 mM) and phosphate (< 1 mM). The assay is not compatible with ammonium, Tris buffer or other primary amine buffers. It is not compatible with amino acids, DTT or EDTA.