Citrate is an important biomolecule and popular additive in pharmaceutical products. The Citrate Assay Kit is for measurement of sub-millimolar to low millimolar concentrations of citrate. The assay is based on increase of the optical density at 490 nm (OD490) of the kit reagent upon binding to citrate. The assay is compatible with Tris-HCl buffers. It is not compatible with phosphate buffers, polycarboxylic acids or other poly acids.
The Citrate Assay kit (catalog number CIT100) includes 1.5 ml of Reagent A, 1.5 ml of 10 x Reagent B and 0.2 ml of 100 mM ammonium citrate. It is for 100 assays using 96-well plates. Cuvettes may also be used for measurements.