Ciprofloxacin is an antibiotic that binds DNA topoisomerases and blocks the DNA replication process in bacteria. The MicroGram Ciprofloxacin Assay Kit is designed for measurement of low micrograms of ciprofloxacin. The assay is based on increase of fluorescence at 470 nm of the assay reagent in the presence of ciprofloxacin. The assay kit can be used for measurement of ciprofloxacin concentrations in drug discovery, drug development and pharmaceutical samples. The assay is not compatible with biological samples containing amino acids or other molecules or buffers with amines.
The MicroGram Ciprofloxacin Assay Kit (Catalog number CIP100) includes 1 ml of 10 x CIP dye, 10 ml of Assay buffer, 10 ml of reagent A. It is for measurement of 100 samples using 96-well plates. Cuvettes may also be used for measurements.