This product includes the reagents for 500 assays in a 384-well plate assay format including 2 ml of 10 x Buffer, 0.170 ml of 100 x UDP-MurNAc-L-Ala (UMA), 0.170 ml of 100 x D-Glu, 0.170 ml of 100 x ATP , 0.170 ml of 100 x E. coli MurD (2000 nM) and 25 ml of Dye for phosphate detection.