Amino acid assay
The Amino Acid Assay Kit (Catalog Number AAK1000) is for measurement of micromolar to millimolar concentrations of amino acids. The assay sensitivity is 0.01 mM for His and Cys and less than 0.5 mM for most amino acids. The assay is based on the principle that amino acids interacts with dye C53 and enhance the fluorescence intensity at 535 nm (excitation at 485 nm).
The assay reagent is sufficient for measurement of 1000 samples using 384-well or 96- well plates. It can also be used for measurement of amino acid concentrations using cuvettes. The assay is compatible with HEPES buffer, low concentrations of non-ionic detergent (<0.01%), MgCl2(< 5 mM), CaCl2 (<5 mM) and phosphate (< 1 mM). The assay is not compatible with Tris buffer or other primary amine buffers. It is not compatible with DTT or EDTA.