Human DNA topoisomerase II and bacterial DNA topoisomerase IV (the parC-parE complex) converts the concatenated DNA into decatenated DNA (decatenation reaction).   Bacterial topoisomerase I and gyrases also have DNA decatenation activity.  The 96-Well Topoisomerase DNA Decatenation Assays are in a 96-well assay plate format that can be used for high-throughput tests of human topo II and bacterial topo IV inhibitors.   The assay is based on the principle that the decatenated DNA is separated from the concatenated DNA by a filtration process.  The decatenated DNA passed through the filter (TDD filter plate), received in a 96 well plate and quantified by fluorescence at 535 nm (excitation at 485 nm).

 96-Well Topoisomerase DNA Decatenation Assay Kit (Catalog No. TDD96K)

 96-Well Human Topo II DNA Decatenation Assay Kit Plus (Catalog No. HDD96KE)

 96-Well E. coli Topo IV DNA Decatenation Assay Kit Plus (Catalog No. EDD96KE)

 96-Well S. aureus Topo IV DNA Decatenation Assay Kit Plus (Catalog No. SDD96KE)

 96-Well S. pneumoniae Topo IV DNA Decatenation Assay Kit Plus (Catalog No. NDD96KE)

 96-Well P. aeruginosa Topo IV DNA Decatenation Assay Kit Plus (Catalog No. PDD96KE)

 96-Well E. coli Topo I DNA Decatenation Assay Kit Plus (Catalog No. T1DD-96KE)   

 96-Well E. coli gyrase DNA Decatenation Assay Kit Plus (Catalog No. T2DD-96KE)  

 96-Well S. aureus gyrase DNA Decatenation Assay Kit Plus (Catalog No. T2DD-96KS)