Beta Nonyl Glucoside Assay

The Beta Nonyl Glucoside (ß-NG) Assay Kit is for measurement of detergent ß-NG at concentrations lower or higher than its CMC value.  The assay is based on the principle that ß-NG interacts with the dye and enhances the fluorescence at 610 nm (Excitation 550 nm). The log values of the fluorescence intensity show a linear relationship with the concentrations of ß-NG.  The assay is compatible with most buffers and salts.   It is not compatible proteins and other detergents. Please use Beta Octyl Glucoside (ß-OG) Assay Kit (Catalog # OG100K) for measurement of ß-OG concentrations; NanoGram SDS Assay Kit (Catalog # SDS200) for measurement of SDS concentrations; and Detergent Assay Kit (Catalog # DAK1000) for other detergents.

The Beta Nonyl Glucoside (ß-NG) Assay Kit includes 0.2 ml of 10 x Dye for NG and 10 ml of dilution buffer.  It is for measurement of 100 samples using 96-well plates. A cuvette may also be used for the measurement of detergent concentrations.