The Detergent Assay Kit (Catalog Number DAK1000) is for detection of various detergents at concentrations below their CMC values for most detergents. The concentration response is characteristic to a specific detergent. The assay is based on the principle that the detergent interacts with dye A43 and enhances the fluorescence intensity at 535 nm (excitation at 485 nm).
The assay reagent (catalog number DAK1000) is sufficient for measurement of 1000 samples using 96-well plates. It can also be used for measurement of detergent concentrations using cuvettes.
The assay is compatible with most buffers and salts. It is not compatible with DTT, phosphate. Molecules that release phosphate may interfere with the assay.