T7 RNA polymerase assay

Bacteriophage T7 RNA polymerase specifically transcribes DNA downstream of a T7 promoter.  The T7 RNA Polymerase Assay Kit is based on measurement of the RNA molecules synthesized by the RNA polymerase using a DNA template including a double strand T7 promoter.  The assay can be performed in a 384-well plate format for tests of T7 RNA polymerase activities and high throughput screening of T7 RNA polymerase inhibitors. 

The T7 RNA Polymerase Assay Kit (enzyme not included) (Catalog number T7RPA100K) includes the assay buffer, DNA template, NTP mix and fluorescence dye for 100 assays of T7 RNA polymerase reactions in a 384-well assay format.  The assay kit includes all reagents except the enzyme.   

The T7 RNA Polymerase Assay Kit Plus (enzyme included) (Catalog number T7RPA100KE) includes the assay buffer, DNA template, NTP mix,T7 RNA polymerase and fluorescence dye for 100 assays of T7 RNA polymerase reactions in a 384-well assay format.

The 10 x Assay Buffer for T7 RNAP assay (Catalog Number: T7BUF-40) includes 40 ml of 10 x assay buffer for T7 RNA polymerase assays.

For enzymes, substrates and templates, please click here.

 

References:

Siegmund V et al, Screening mutant libraries of T7 RNA polymerase for candidates with increased acceptance of 2'-modified nucleotides, Chem. Commun., 48, 9870-9872 (2012).