Alcohol dehydrogenase (ADH) is a popular enzyme present in most organisms. It catalyzes the oxidation of alcohols to form aldehydes or ketones.  It not only plays key roles in biological detoxification of alcohol in human but also actively involves in the generation of aldehydes and ketones during the biosynthesis of various metabolites. The ADH reaction is reversible and is broadly used in industry to synthesize chiral pharmaceuticals and other molecules for various applications. The alcohol dehydrogenase assay is colorimetric and high throughput. The assay is based on detection of NADH by light absorbance at 590 nm using the NADH assay kit (Catalog No. NADH100).

Primary Alcohol     +  NAD⁺    =   Aldehyde + NADH + H⁺

Secondary Alcohol +  NAD⁺    =    Ketone + NADH + H⁺

The Alcohol Dehydrogenase Assay Kit (Catalog No. ADH100K) includes 500 µl of 10 x Buffer, 300 µl of 10 x Ethanol (20%), 300 µl of 10 x NAD⁺(2 mM), 120 µl of 10x Reagent A. 120 µl of 10 x Reagent B and 150 µl of 10 x Reagent C. It is for 100  assays in a 384-well plate assay format or 50 assays in a 96-well plate. Enzyme is not included in the kit.

The Yeast Alcohol Dehydrogenase Assay Kit Plus (Catalog No. ADH100KY)  includes all the reagents in the Alcohol Dehydrogenase Assay Kit (Catalog No. ADH100K) plus 10 µl of 300 x yeast Alcohol Dehydrogenase.