The Alcohol Dehydrogenase Assay Kit (Catalog No. ADH100K) includes 500 µl of 10 x Buffer, 300 µl of 10 x Ethanol (20%), 300 µl of 10 x NAD⁺(2 mM), 120 µl of 10x Reagent A. 120 µl of 10 x Reagent B and 150 µl of 10 x Reagent C. It is for 100  assays in a 384-well plate assay format or 50 assays in a 96-well plate. Enzyme is not included in the kit.