Each 96-Well Topoisomerase DNA Decatenation Assay Kit (Catalog No. TDD96K)includes 600 µl of 10 x assay buffer, 500 µl of 10 x concatenated DNA, 110 µl of 10 mM ATP, 600 µl of 0.4 M EDTA, 260 µl of 20 x fluorescence dye, 2 ml of 10 x rinse buffer, one V-bottom plate, a TDD filter plate and one black 96-well plates for 96 assays of DNA decatenation reactions. The assay buffer is optimized for bacterial topoisomerase IV. DNA decatenation enzyme is not included. This kit can be used for DNA decatenation assays of any bacterial topoisomerase IV (the parC-parE complex) enzymes or gyrases.