Viral RNA-dependent RNA Polymerase Assay

The Viral RNA-dependent RNA Polymerase Assay is developed using a RNA polymerase in the Flaviviridae, a family of positive, single-stranded, enveloped RNA viruses. The assay is based on measurement of the RNA molecules synthesized by the RNA polymerase using RNA as a template in the presence of NTPs.  The assay can be performed in a 384-well or 96-well plate format for tests of theenzyme activities of RNA polymerases in the Flaviviridae family and high throughput screening of inhibitors.  

The Viral RNA-dependent RNA Polymerase Assay Kit – 100 (Catalog No.VRT100K) includes 350 µl of 10 x Buffer, 33 µl of 100 x Template, 33 µl of 100 x NTPs (50 mM ATP and 50 mM GTP) and 330 µl of 10 x fluorescence dye. It is for 100 assays of virus RNA polymerase in a 384-well plate format. The assay kit includes all reagents except the enzyme.   

The Viral RNA-dependent RNA Polymerase Assay Kit – 500 (Catalog No.VRT500K) includes 1550 µl of 10 x Buffer, 160 µl of 100 x Template, 160 µl of 100 x NTPs (50 mM ATP and 50 mM GTP) and 1550 µl of 10 x fluorescence dye. It is for 500 assays of virus RNA polymerase in a 384-well plate format. The assay kit includes all reagents except the enzyme.