The Topo World includes various non-radioactive and high-throughput assays for all topoisomerase functions including DNA relaxation, DNA supercoiling, DNA decatenation and ATP hydrolysis. It also includes DNA cleavage assays for type II topoisomerases including topo II and topo IV in the presence of certain topoisomerase drugs (topoisomerase poisons). The assays are formatted for topoisomerases including human topo I, human topo II, gram-negative and gram-positive bacterial topo I, topo II (gyrase) and topo IV (parC-parE complex). Furthermore, the principle of the DNA relaxation, supercoiling and decatenation assays are universal and can be used for DNA topoisomerases from any species. These assays can be used for topoisomerase characterization, drug tests and high-throughput screening (HTS) of topoisomerase inhibitors for drug discovery and research.
High throughput DNA topoisomerase assays
General topoisomerase assays
Human topoisomerase assays
Bacterial topoisomerase assays
Gel-Based DNA topoisomerase assays
ProFoldin also offers traditional gel-based DNA supercoiling, DNA relaxation and DNA decatenation assay kits for customers to visualize the DNA topoisomerase products on agarose gels.
Gel-based topoisomerase DNA supercoiling assays
Gel-based topoisomerase DNA relaxation assays
Gel-based topoisomerase DNA decatenation assays
Topoisomerase enzymes and DNA substrates
Relaxed plasmid DNA - 0.050 mg
Supercoiled plasmid DNA - 0.050 mg
Topoisomerase enzymes