RNA polymerase assays

 

The following RNA polymerase assays in a 384-well plate format are available:

 

Bacterial RNA polymerase assays

Two types of bacterial RNAP assays are available: one is to measure RNA synthesis, the other is to measure DNA -dependent pyrophosphate formation.

            E. coli RNA polymerase assay: A fluorescence-based assay to measure RNA synthesis by E. coli RNA polymerase using a single strand DNA template.

            E. coli RNA polymerase pyrophosphate assay: A light absorbance-based assay to measure pyrophosphate formation during RNA formation by E. coli RNA polymerase using a double-strand DNA template.

            S. aureus RNA polymerase assay: A fluorescence-based assay to measure RNA synthesis by S. aureus RNA polymerase using a single strand DNA template.

            S. aureus RNA polymerase pyrophosphate assay: A light absorbance-based assay to measure pyrophosphate formation during RNA formation by S. aureus RNA polymerase using a double-strand DNA template.

            E. coli Polynucleotide Phosphorylase (PNPase) Assay: A fluorescence-based assay to measure RNA synthesis by E. coli PNPase using an RNA template.

 

Bacteriophage T7 RNA polymerase assay

         A fluorescence-based assay to measure RNA synthesis by T7 RNA polymerase using a DNA template composed of a double strand T7 promoter sequence with an extension of a single strand DNA chain.