Protein stability and aggregation assay

The Protein Stability and Aggregation Assay Kit is for evaluation of protein thermal stability and analysis of protein unfolding aggregation. The assay is based on a fluorescence dye binding to the hydrophobic surfaces of the proteins and generates fluorescence at 610 nm. Since the unfolded (denatured) proteins have more hydrophobic surfaces than the native ones, the unfolded protein generates a higher fluorescence intensity than the native protein does at the same protein concentration in the same buffer.   By incubation of the native protein at a raised temperature, a thermal unfolding curve can be observed and the half life time (t1/2) of the protein is calculated. The half life time value can be used to evaluate protein stability such as comparison between the wild-type and mutant proteins.   The thermal unfolding curve can also be used to analyze the protein unfolding aggregation state of protein samples.

Protein stability and aggregation assay kit (catalog number PSA200K)

Each kit (Catalog number PSA200K) contains the 1000 x PSA dye for 200 assays with the assay volume of 0.3 ml using 96-well plates or cuvettes.
 

References:

  1. Takahashi N et al, TRPV4 channel activity is modulated by direct interaction of the ankyrin domain to PI(4,5)P₂. Nat Commun. 26;5:4994 (2014).
  2. Srinivasan S et al, Hypoxia-induced expression of phosducin-like 3 regulates expression of VEGFR-2 and promotes angiogenesis. Angiogenesis 18:449–462 (2015).