Protein assay

Easy Protein Assay Reagent     (catalog EPA001)

The Assay Reagent is based on the principle of Bradford method.  Proteins bind to Coomassie Brilliant Blue in an acidic solution and absorb the light at 595 nm wavelength.  The assay is quick and easy:

1. Mix 0.2 ml of the reagent with 0.020 ml of the protein solution.

2. Read absorbance at 595 nm.

 

Standard clear 96-well plates or cuvettes can be used for absorbance reading.  The reagent is sufficient for 2500 assays (0.2 ml / assay using micro plate) or 500 assays (1 ml / assay using 1 ml-cuvette).