Polyphosphate assay

Inorganic polyphosphate is a linear molecule composed of tens or hundreds of phosphate residues linked together.  In bacteria, polyphosphate kinase (PPK) converts polyphosphate and ADP to ATP.   The MicroMolar Polyphosphate Assay Kit is for measurement of micromolar concentrations of polyphosphate.  The assay is based on increase of the fluorescence intensity (emission 550 nm, excitation 415 nm) of the kit fluorescence dye PPD upon binding to polyphosphate.  The assay is compatible with regular buffers and various phosphate compounds including inorganic phosphate, pyrophosphate, ATP, ADP and AMP.  The assay kit can be used for measurements of polyphosphate in biological samples or environmental water samples.  

MicroMolar Polyphosphate Assay Kit  (Catalog number: PPD1000)

The MicroMolar Polyphosphate Assay Kit is for measurement of micromolar concentrations of polyphosphate.  The assay is based on increase of the fluorescence intensity (emission 550 nm, excitation 415 nm) of the kit fluorescence dye PPD upon binding to polyphosphate.  The assay is compatible with regular buffers and various phosphate compounds including inorganic phosphate, pyrophosphate, ATP, ADP and AMP.  The assay kit can be used for measurements of polyphosphate in biological samples or environmental water samples.  

References:

  1. Achbergerová.L et al, Degradation of polyphosphates by polyphosphate kinases from Ruegeria pomeroyi. Biotechnology Letters. Volume 36, Issue 10, pp 2029-2035 (2014).
  2. Zhang J et al, A Fast Sensor For in Vivo Quantification of Cytosolic Phosphate in Saccharomyces Cerevisiae, Biotechnol Bioeng.Vol 112, pp 1033-1046 (2015).